Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
Continue reading...
，这一点在体育直播中也有详细论述
Étienne Fortier-Dubois
更多精彩内容，关注钛媒体微信号（ID：taimeiti），或者下载钛媒体App
Review coverage effect: But when they do introduce bugs, there are fewer reviewers around to catch them quickly. Monday morning reviewers are triaging a backlog, not scrutinizing each weekend patch.